Tn5563, a transposon encoding putative mercuric ion transport proteins located on plasmid pRA2 of Pseudomonas alcaligenes
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چکیده
منابع مشابه
Characterization of the endogenous plasmid from Pseudomonas alcaligenes NCIB 9867: DNA sequence and mechanism of transfer.
The endogenous plasmid pRA2 from Pseudomonas alcaligenes NCIB 9867 was determined to have 32,743 bp with a G+C content of 59.8%. Sequence analysis predicted a total of 29 open reading frames, with approximately half of them contributing towards the functions of plasmid replication, mobilization, and stability. The Pac25I restriction-modification system and two mobile elements, Tn5563 and IS1633...
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The nonconjugative plasmid, pVS1, has a molecular weight of 18.5 X 10(6) and confers resistance to sulfonamides and to mercuric ions. In Pseudomonas aeruginosa PAO, the transfer can be mobilized by a variety of conjugative plasmids, and the process does not require a functional recombination system in the donor. Hybrid plasmids that arise by the relocation of the mer gene onto the mobilizing pl...
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After freezing and thawing, Pseudomonas aeruginosa harboring a drug resistance plasmid (Hg , Strep ), became acutely sensitive to mercuric ions but not to streptomycin in the plating medium. Its sensitivity to both agents in the plating medium became more pronounced, suggesting a synergistic effect of the mercuric ions with streptomycin. This freeze-thaw-induced sensitivity was transient and ca...
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After freezing and thawing, Pseudomonas aeruginosa harboring a drug resistance plasmid (Hg2+r, Strr), became acutely sensitive to mercuric ions but not to streptomycin in the plating medium, whereas its sensitivity to both agents became more pronounced indicating a synergistic effect. This freeze-thaw-induced sensitivity was transient and capable of being repaired to a simple salts medium. Tran...
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Pseudomonas sp. strain ADP initiates atrazine catabolism via three enzymatic steps, encoded by atzA, -B, and -C, which yield cyanuric acid, a nitrogen source for many bacteria. In-well lysis, Southern hybridization, and plasmid transfer studies indicated that the atzA, -B, and -C genes are localized on a 96-kb self-transmissible plasmid, pADP-1, in Pseudomonas sp. strain ADP. High-performance l...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1998
ISSN: 0378-1097
DOI: 10.1016/s0378-1097(98)00286-9